Biotinylated Human FOLR1 Protein C-His-Avi Tag | Syd Labs BP005635

Biotinylated Human FOLR1 Protein BP005635 Syd Labs

Biotinylated Human FOLR1 Protein C-His-Avi Tag | Syd Labs BP005635

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(悉得)Syd Labs 货号:BP005635 Biotinylated Human FOLR1 Protein with the C-His-Avi Tag (生物素化人 FOLR1 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg25-Met233.

SKU: BP005635 分类: ,

产品参数

货号 BP005635
产品名称Biotinylated Human FOLR1 Protein C-His-Avi Tag | Syd Labs BP005635
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18
物种 Human
来源 The recombinant Biotinylated Human FOLR1 Protein, (amino acids Arg25-Met233) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-His-Avi
生物素化 yes
确定序列 Arg25-Met233
分子量 The recombinant Biotinylated Human FOLR1 protein with the His-Avi tag at the C-terminus has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Anti-FOLR1 Antibody protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human FOLR1 protein with the C-His tag with the EC50 of 13.9 ng/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Biotinylated Human FOLR1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Biotinylated Human FOLR1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Biotinylated Human FOLR1 Protein performance should be determined experimentally by the investigator.
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文献

BP005635: Biotinylated Human FOLR1 Protein with the C-His-Avi Tag (生物素化人 FOLR1 蛋白 C-His-Avi 标签)

 

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 – 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.

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