UniMab 50 HC Protein A Resin

描述

IA122: UniMab 50 HC Protein A Resin

Background

Our alkaline-stable recombinant protein A ligands are immobilized onto our proprietary polymethacrylate base matrices to provide cost-effective solutions for fast-processing of monoclonal and polyclonal antibodies, bispecific antibodies, and Fc fusion constructs with high quality.

IA122: UniMab 50 HC Protein A Affinity Resin

UniMab® 50 HC protein A resin is a high-performance, alkali-resistant recombinant Protein A affinity chromatography medium developed by NanoMicro using its proprietary technologies. The resin is made with monodisperse, crosslinked polymethacrylate matrix, proprietary surface modification and epoxy coupling chemistry for bonding Protein A. It is suitable for the separation and purification of monoclonal antibodies and recombinant protein biological macromolecules containing Fc domains. UniMab® 50 HC has high mechanical strength, low back pressure, good chemical stability, and strong alkali resistance. Even at high flow rates, it can still maintain a high dynamic adsorption capacity, which can significantly improve productivity for laboratory preparation to pilot test and industrial production. Table 1 lists the basic characteristics of this protein A resin:

Characteristics: Specification
Separation Function: Protein A affinity
Matrix: Monodisperse, crosslinked polymethacrylate
Ligand: Alkaline-stabilized recombinant protein A
Coupling Chemistry: Epoxy
Particle Size（μm): average 50 μm
Dynamic Binding Capacity: 50 mg/mL (human IgG, 4 minute residence time)
Protein A Leachate: typically < 10 ppm Maximum Pressure: 0.8 MPa Clean in Place: 0.1-0.5 M NaOH Recommended Flow Velocity: <600 cm/hour pH Stability: 3-12 Chemical Stability: Stable in commonly used buffers, 0.1 M HAc, 0.1 M HCl, 100% ethanol, isopropyl alcohol, etc. Avoid long-term exposure to strong acids or strong bases. Operational Temperature: 2-40 ℃ Storage: 20% Ethanol or benzyl alcohol, 2-8 ℃ The engineered protein A in UniMab® 50 HC protein A affinity resin is manufactured with animal free raw materials. UniMab® 50 HC protein A affinity resin provides reliable all around performance, low protein A leakage, high binding capacity and a useful effective working range requiring a residence time of at least 3 minutes (equivalent to a typical running speed of 300 cm/hr). It is available in bottled (20% ethanol at 60% slurry concentration) or pre-packed column packaging. For comparison, please see:

NMab protein A affinity resin
NMab Pro Protein A affinity resin

UniMab 50 HC protein A affinity resin, which combines the monodisperse, crosslinked polymethacrylate and the engineered protein A ligand using proprietary technologies of crosslinking, surface extension, and ligand coupling, is the major component in pre-packed protein A columns.

Please remember our product information: UniMab 50 HC Protein A Resin: IA122 Syd Labs