RNase Inhibitor

描述

MB079: Recombinant Murine RNase Inhibitor (RI)

Background

Recombinant RNase inhibitors (ribonuclease inhibitors, RI) are used to inhibit RNase activity during RNA-related experiments. Small amounts of RNase may contaminate the purified RNA during most of RNA extraction procedures, and compromise downstream applications such as reverse transcription (RT), RT-PCR, and RT-qPCR. Such contamination can also be introduced via tips, tubes, and other reagents used in these downstream applications. Mammalian ribonuclease inhibitors, ~50-kDa cytosolic proteins, bind to pancreatic-type ribonucleases with femtomolar affinity and effectively prevent RNase contamination. The murine RNase inhibitor has better resistance to oxidation than the human and porcine RNase inhibitors, which is ideal for RT-PCR and RT-qPCR reactions where low DTT concentrations are required.

The recombinant murine RNase inhibitor was expressed in and purified from E. coli. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with various Reverse Transcriptases and Taq DNA Polymerases. Neither DNases nor RNases are present.

Unit definition: One unit of the recombinant murine RNase inhibitor means the protein amount used to inhibit the activity of 5 ng of RNase A by 50%, which is measured by the inhibition of hydrolysis of cytidine 2′, 3′-cyclic monophosphate by RNase A.

Concentration: 40 units/ul.

Shipping: The recombinant murine RNase inhibitor is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 year from date of receipt, -20°C to -70°C as supplied.

Features of the Recombinant Murine RNase Inhibitor:
* High purity;
* Neither DNases nor RNases present;
* Compatible with RT-PCR and RT-qPCR.

Applications of the Recombinant Murine RNase Inhibitor:
* In vitro transcription (mRNA synthesis) and translation;
* cDNA synthesis (reverse transcription, RT);
* RT-PCR and RT-qPCR;
* RNA labeling.

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR PreMix with dye (green, red, blue).
* 2x rapid PCR Master Mix with a green dye.
* 2x long PCR Master Mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT-PCR master mixes
* 2x qPCR master mixes
* RT-qPCR master mixes

PCR components and related products:
* dNTP mix, 10 mM each.
* dNTP mix, 25 mM each.
* Proteinase K powder .
* Proteinase K solution.
* Reverse Transcriptases.
* First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* PCR enhancer.

Please remember our product information: RNase Inhibitor: MB079 Syd Labs