Recombinant Heparinase III Enzyme
Syd Labs Recombinant Heparinase III Enzyme BP000033-GD7, Different from Heparinases I and II, Heparinase III (EC 4.2.2.8) cleaves the glycosidic bond between hexosamines and glucuronic acid residues only which is present in heparan sulfate rather than heparin. Specific activity of recombinant Heparinase III >50 IU/mg.
- 产品参数
- 文献
- More Offers
| 货号 | BP000033-GD7 |
|---|---|
| 产品名称 | Recombinant Heparinase III Enzyme |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | Heparin Lyase III |
| 来源 | E. coli-derived. |
| 形式 | In hepes buffer with 30% glycerol and 0.01 M Ca2+. |
| 纯度 | >95% by SDS-PAGE. |
| 运输 | Recombinant heparinase III is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 注意事项 | Conditions of optimal Heparinase III Enzyme performance should be determined experimentally by the investigator. Custom service to make mixture of Heparinases (I, II, and/or III) is available. |
| 线下下单 | 电话: 18162660842微信: duozhaozhao_2017Email: message@sydlabs.comOr 联系我们 |
描述
BP000033-GD7: Recombinant Heparinase III Enzyme
Background
Different from heparinase I and heparinase II, Heparinase III enzyme (EC 4.2.2.8) cleaves the glycosidic bond between hexosamines and glucuronic acid residues in non-sulfated regions only which is present in heparan sulfate rather than heparin.
Similar to heparin, located in the extracellular matrix, the glycosaminoglycan of heparan plays important physiological roles in anticoagulation and angiogenesis. The acidic polysaccharide of heparan consists of a heterogeneous disaccharide repeating unit of hexosamine and uronic acid (L-iduronic or D-glucuronic acid) connected through 1-4 linkages and modified with various functional groups. Sulfated regions of heparan sulfate are interspaced with less or non-sulfated regions but heparin sulfate contains no non-sulfated regions.
Source: E. coli-derived.
Specific activity >50 IU/mg. One international unit (IU) of recombinant Heparinase III is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparan sulfate per minute at 30°C and pH 7.3.
Purity >95% by SDS-PAGE.
Molecular weight: 70,800.
Isoelectric point: 9.9 – 10.1.
Form: In hepes buffer with 30% glycerol and 0.01 M Ca2+.
Shipping: Recombinant heparinase III is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
Please remember our product information: Recombinant Heparinase III Enzyme: BP000033-GD7 Syd Labs