Recombinant Endotoxin-free TEV Protease

Recombinant Endotoxin-free TEV Protease: BP4114 Syd Labs

Recombinant Endotoxin-free TEV Protease

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Syd Labs Recombinant Endotoxin-free TEV Protease BP4114, Recombinant endotoxin-free TEV protease cleaves after Gln in the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln.

SKU: BP4114 分类: ,
货号 BP4114
产品名称Recombinant Endotoxin-free TEV Protease
供货商名称 Syd Labs, Inc.
品牌名 悉得(Syd Labs)
别称 NIa
来源 E.coli-derived.
形式 0.2 μM filtered solution.
纯度 >95% by SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU/ug of protein as determined by LAL method.
运输 The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
注意事项 Conditions of optimal endotoxin-free TEV protease performance should be determined experimentally by the investigator.
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描述

BP4114: Recombinant Endotoxin-free TEV Protease

Background

The TEV protease, also designated NIa, is 241 amino acids long and is the catalytic domain of the encoded potyvirus protein. Endotoxin-free TEV protease is effectively used with high specificity and with various pHs and ionic strengths as a preferable protease to cleave affinity tags from recombinant proteins. This TEV protease can be used either co-translationally or following purification in vitro.

BP4114: Recombinant endotoxin-free TEV protease

Source: E.coli-derived.
Recombinant endotoxin-free TEV protease is the sequence of TEV Protease (accession # NP_062908, amino acids Glu2039-Gln2279 (Ser2256Asn)) fused with a 6-His tag at the C-terminus.
Purity: >95% by SDS-PAGE under reducing conditions.
Activity: Recombinant endotoxin-free TEV protease cleaves after Gln in the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln.
Formulation: 0.2 μM filtered solution.
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Please remember our product information: Recombinant Endotoxin-free TEV Protease: BP4114 Syd Labs

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