Rat Coagulation Factor II Protein C-His Tag | Syd Labs BP003275

Rat Coagulation Factor II Protein BP003275 Syd Labs

Rat Coagulation Factor II Protein C-His Tag | Syd Labs BP003275

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Syd Labs 货号:BP003275 Rat Coagulation Factor II Protein with the C-His Tag (大鼠凝血因子 II 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-terminal.It contains Gln25-Arg617.

SKU: BP003275 分类: ,

产品参数

货号 BP003275
产品名称Rat Coagulation Factor II Protein C-His Tag | Syd Labs BP003275
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
物种 Rat
来源 The recombinant Rat Coagulation Factor II Protein, (amino acids Gln25-Arg617) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-His
生物素化 no
确定序列 Gln25-Arg617
分子量 The recombinant Rat Coagulation Factor II protein with the His tag at the C-terminus has a predicted MW of 69.12 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Rat Coagulation Factor II Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Rat Coagulation Factor II Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Rat Coagulation Factor II Protein performance should be determined experimentally by the investigator.
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文献

BP003275: Rat Coagulation Factor II Protein with the C-His Tag (大鼠凝血因子 II 蛋白 C-His 标签)

 

Prothrombin, or coagulation factor II, is a multidomain zymogen precursor of thrombin that undergoes an allosteric equilibrium between two alternative conformations, open and closed, that react differently with the physiological activator prothrombinase. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites.

请记住我们的产品信息: 大鼠凝血因子 II 蛋白 (C-His 标签)BP003275 Syd Labs(Rat Coagulation Factor II Protein with the C-His Tag).