Plasmid Maxiprep Kits (EndoFree, Spin-column)

描述

MB074-PL: EndoFree Plasmid Maxiprep Kit (Spin-column)

Background

The spin column-based endoFree plasmid maxiprep kit is designed for rapid purification of high-quality large-amount endofree plasmid DNA ready for applications such as DNA sequencing, DNA cloning, and DNA transfection. The kit applies the improved alkaline-SDS lysis method to lyses cells rapidly. The endotoxin in crudely extracted samples can be selectively removed by simple separation. Then DNA is selectively absorbed on silica membrane in the spin column under the high salt condition and eluted under the low salt condition.

Features of the endoFree plasmid maxiprep kit (spin-column):

Up to 1.5 mg yield of plasmid DNA from 150 mL bacterial overnight culture.
Endotoxin level <0.1 EU/ug Rapid and convenient. No poisonous phenol nor ethanol precipitation. Multi-elution can ensure high-purified DNA, which can be applied to all kinds of molecular experiments such as DNA transfection, sequencing, cloning, PCR, Southern-blot, and enzymatic digestion directly. Please remember our product information: EndoFree Plasmid Maxiprep Kit (Spin-column): MB074-PL Syd Labs