Mouse IL-17F Protein C-His-Avi Tag | Syd Labs BP004287
(悉得)Syd Labs 货号:BP004287 Mouse IL-17F Protein with the C-His-Avi Tag (小鼠 IL-17F 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg29-Ala161.
产品参数
| 货号 | BP004287 |
|---|---|
| 产品名称 | Mouse IL-17F Protein C-His-Avi Tag | Syd Labs BP004287 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Cytokine ML-1; IL17F; IL-17F; IL24; IL-24; CANDF6; IL-17F; ML1 |
| 物种 | Mouse |
| 来源 | The recombinant Mouse IL-17F Protein, (amino acids Arg29-Ala161) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q7TNI7-1 |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Arg29-Ala161 |
| 分子量 | The recombinant Mouse IL-17F protein with the His-Avi tag at the C-terminus has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Mouse IL-17F protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Mouse IL-17R alpha protein with the C-hFc tag with the EC50 of 0.2 ug/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Mouse IL-17F Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1EU per ug by the LAL method. |
| 运输 | The recombinant Mouse IL-17F Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Mouse IL-17F Protein performance should be determined experimentally by the investigator. |
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文献
BP004287: Mouse IL-17F Protein with the C-His-Avi Tag (小鼠 IL-17F 蛋白 C-His-Avi 标签)
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover.
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