[KO Validated] Cyclin D1 Rabbit mAb (A19038)

描述

A19038: [KO Validated] Cyclin D1 Rabbit mAb

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

Immunogen Information about [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunogen： A synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385).
Sequence： ISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
Gene ID： 595
Swiss prot： P24385
Synonyms： BCL1; PRAD1; U21B31; D11S287E; D1
Calculated MW： 34kDa
Observed MW： 34kDa

Images of [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of lysates from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Western blot analysis of various lysates using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Immunohistochemistry analysis of Cyclin D1 in paraffin-embedded human colon carcinoma using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of Cyclin D1 in paraffin-embedded mouse colon using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (A19038, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Please remember our product information: [KO Validated] Cyclin D1 Rabbit mAb (Catalog Number: A19038) (悉得）Syd Labs