Human VEGF R1/FLT-1 Protein C-His-Avi Tag | Syd Labs BP005456
(悉得)Syd Labs 货号:BP005456 Human VEGF R1/FLT-1 Protein with the C-His-Avi Tag (人 VEGF R1/FLT-1 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser27-Asn756.
产品参数
| 货号 | BP005456 |
|---|---|
| 产品名称 | Human VEGF R1/FLT-1 Protein C-His-Avi Tag | Syd Labs BP005456 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | FLT; FLT1; Flt-1; FRT; VEGF R1; VEGFR1 |
| 物种 | Human |
| 来源 | The recombinant Human VEGF R1/FLT-1 Protein, (amino acids Ser27-Asn756) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Ser27-Asn756 |
| 分子量 | The recombinant Human VEGF R1/FLT-1 protein with the His-Avi tag at the C-terminus has a predicted MW of 85.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Human PGF protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human VEGF R1 protein with the C-His tag with the EC50 of 12.8 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human VEGF R1/FLT-1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human VEGF R1/FLT-1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human VEGF R1/FLT-1 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品,不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题,欢迎随时联系Syd Labs国内市场推广合作伙伴:武汉多找找科技有限公司,企业微信:duozhaozhao2024 联系电话:18162581039(龙经理) |
文献
BP005456: Human VEGF R1/FLT-1 Protein with the C-His-Avi Tag (人 VEGF R1/FLT-1 蛋白 C-His-Avi 标签)
VEGFR1 (vascular endothelial growth factor receptor 1), also called Flt-1 (Fms-like tyrosine kinase), is a 180 kDa type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTKs).yrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells.
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