Human VCAM-1/CD106 Protein C-His Tag | Syd Labs BP005039
(悉得)Syd Labs 货号:BP005039 Human VCAM-1/CD106 Protein with the C-His Tag (人 VCAM-1/CD106 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Pro697.
产品参数
| 货号 | BP005039 |
|---|---|
| 产品名称 | Human VCAM-1/CD106 Protein C-His Tag | Syd Labs BP005039 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | V-CAM 1; VCAM-1; INCAM-100; CD106; DKFZp779G2333; MGC99561 |
| 物种 | Human |
| 来源 | The recombinant Human VCAM-1/CD106 Protein, (amino acids Phe25-Pro697) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | NP_001069 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Phe25-Pro697 |
| 分子量 | The recombinant Human VCAM-1/CD106 protein with the His tag at the C-terminus has a predicted MW of 75.2 kDa. Due to glycosylation, the protein migrates to 75-110 kDa based on Bis-Tris PAGE result. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human VCAM-1/CD106 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human VCAM-1/CD106 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human VCAM-1/CD106 Protein performance should be determined experimentally by the investigator. |
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文献
BP005039: Human VCAM-1/CD106 Protein with the C-His Tag (人 VCAM-1/CD106 蛋白 C-His 标签)
Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.
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