Human TEM1/cd248 Protein C-His Tag | Syd Labs BP004884
(悉得)Syd Labs 货号:BP004884 Human TEM1/cd248 Protein with the C-His Tag (人 TEM1/cd248 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Leu687.
产品参数
| 货号 | BP004884 |
|---|---|
| 产品名称 | Human TEM1/cd248 Protein C-His Tag | Syd Labs BP004884 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Endosialin; CD248; CD164L1; TEM1; CD164 sialomucin-like 1; CD164L1; CD248 molecule; MGC119479 |
| 物种 | Human |
| 来源 | The recombinant Human TEM1/cd248 Protein, (amino acids Gln18-Leu687) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q9HCU0-1 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Gln18-Leu687 |
| 分子量 | The recombinant Human TEM1/cd248 protein with the His tag at the C-terminus has a predicted MW of 72.7 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Human TEM1 protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-TEM1 Antibody protein with the C-hFc tag with the EC50 of 2.0 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human TEM1/cd248 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human TEM1/cd248 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human TEM1/cd248 Protein performance should be determined experimentally by the investigator. |
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文献
BP004884: Human TEM1/cd248 Protein with the C-His Tag (人 TEM1/cd248 蛋白 C-His 标签)
Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC; TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9.
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