Human RANKL/TNFSF11/CD254 Protein N-His-Flag Tag | Syd Labs BP004797
(悉得)Syd Labs 货号:BP004797 Human RANKL/TNFSF11/CD254 Protein with the N-His-Flag Tag (人 RANKL/TNFSF11/CD254 蛋白 N-His-Flag 标签) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gly63-Asp244.
产品参数
| 货号 | BP004797 |
|---|---|
| 产品名称 | Human RANKL/TNFSF11/CD254 Protein N-His-Flag Tag | Syd Labs BP004797 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2 |
| 物种 | Human |
| 来源 | The recombinant Human RANKL/TNFSF11/CD254 Protein, (amino acids Gly63-Asp244) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells. |
| Accession | O14788-2 |
| 产品标签 | N-His-Flag |
| 生物素化 | no |
| 确定序列 | Gly63-Asp244 |
| 分子量 | The recombinant Human RANKL/TNFSF11/CD254 protein with the His-Flag tag at the N-terminus has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human RANKL protein with the N-His-Flag tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A protein with the N-hFc tag with the EC50 of 17.4 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human RANKL/TNFSF11/CD254 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | > 90% as determined by Tris-Bis PAGE |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human RANKL/TNFSF11/CD254 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human RANKL/TNFSF11/CD254 Protein performance should be determined experimentally by the investigator. |
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文献
BP004797: Human RANKL/TNFSF11/CD254 Protein with the N-His-Flag Tag (人 RANKL/TNFSF11/CD254 蛋白 N-His-Flag 标签)
Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.
请记住我们的产品信息: 人 RANKL/TNFSF11/CD254 蛋白 (N-His-Flag 标签)BP004797 Syd Labs(Human RANKL/TNFSF11/CD254 Protein with the N-His-Flag Tag).



