Human R spondin 1/RSPO1 Protein C-His Tag | Syd Labs BP005579
(悉得)Syd Labs 货号:BP005579 Human R spondin 1/RSPO1 Protein with the C-His Tag (人 R spondin 1/RSPO1 蛋白 C-His 标签) is expressed from CHO with His tag at the C-Terminus.It contains Arg31-Ala263.
产品参数
| 货号 | BP005579 |
|---|---|
| 产品名称 | Human R spondin 1/RSPO1 Protein C-His Tag | Syd Labs BP005579 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO |
| 物种 | Human |
| 来源 | The recombinant Human R spondin 1/RSPO1 Protein, (amino acids Arg31-Ala263) fused with a His tag at the C-terminus, was produced in CHO cells. |
| Accession | Q2MKA7 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Arg31-Ala263 |
| 分子量 | The recombinant Human R spondin 1/RSPO1 protein with the His tag at the C-terminus has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human R spondin 1 protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-R spondin 1 Antibody protein with the C-hFc tag with the EC50 of 2.9 ng/ml determined by ELISA (QC Test). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human R spondin 1/RSPO1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human R spondin 1/RSPO1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human R spondin 1/RSPO1 Protein performance should be determined experimentally by the investigator. |
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文献
BP005579: Human R spondin 1/RSPO1 Protein with the C-His Tag (人 R spondin 1/RSPO1 蛋白 C-His 标签)
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated ‘thrombin-sensitive protein.
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