Human PGF Protein C-hFc Tag | Syd Labs BP004811

Human PGF Protein C-hFc Tag BP004811 Syd Labs

Human PGF Protein C-hFc Tag | Syd Labs BP004811

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Syd Labs 货号:BP004811 Human PGF Protein with the C-hFc Tag (人 PGF 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu19-Arg221.

SKU: BP004811 分类: ,

产品参数

货号 BP004811
产品名称Human PGF Protein C-hFc Tag | Syd Labs BP004811
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2
物种 Human
来源 The recombinant Human PGF Protein, (amino acids Leu19-Arg221) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-hFc
生物素化 no
确定序列 Leu19-Arg221
分子量 The recombinant Human PGF protein with the hFc tag at the C-terminus has a predicted MW of 48.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human PGF protein with the C-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human VEGF R1 protein with the C-His tag with the EC50 of 12.8 ng/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human PGF Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human PGF Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Human PGF Protein performance should be determined experimentally by the investigator.
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文献

BP004811: Human PGF Protein with the C-hFc Tag (人 PGF 蛋白 C-hFc 标签)

 

Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients.

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