Human Notch 3 Protein C-His Tag | Syd Labs BP003774
(悉得）Syd Labs 货号：BP003774 Human Notch 3 Protein with the C-His Tag (人 Notch 3 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Ala1378-Ser1640.
产品参数
| 货号 | BP003774 |
|---|---|
| 产品名称 | Human Notch 3 Protein C-His Tag | Syd Labs BP003774 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Human |
| 来源 | The recombinant Human Notch 3 Protein, (amino acids Ala1378-Ser1640) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q9UM47 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Ala1378-Ser1640 |
| 分子量 | The recombinant Human Notch 3 protein with the His tag at the C-terminus has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human Notch 3 protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-Notch 3 Antibody protein with the C-hFc tag with the EC50 of 3.8 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Notch 3 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Notch 3 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human Notch 3 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP003774: Human Notch 3 Protein with the C-His Tag (人 Notch 3 蛋白 C-His 标签)
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
请记住我们的产品信息: 人 Notch 3 蛋白 （C-His 标签）BP003774 Syd Labs（Human Notch 3 Protein with the C-His Tag）.
