Human MMP-9 Protein BP005636 Syd Labs
Human MMP-9 Protein (MMP-HM409)
Human MMP-9 Protein (MMP-HM409)
Human MMP-9 Protein BP005636 Syd Labs
Human MMP-9 Protein (MMP-HM409)
Human MMP-9 Protein (MMP-HM409)

Human MMP-9 Protein C-His-Avi Tag | Syd Labs BP005636

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Human MMP-9 Protein BP005636 Syd Labs

Human MMP-9 Protein C-His-Avi Tag | Syd Labs BP005636

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(悉得）Syd Labs 货号：BP005636 Human MMP-9 Protein with the C-His-Avi Tag (人 MMP-9 蛋白 c-his-avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Ala20-Asp707.

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SKU： BP005636 分类： ,

产品参数

货号 BP005636
产品名称Human MMP-9 Protein C-His-Avi Tag | Syd Labs BP005636
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
物种 Human
来源 The recombinant Human MMP-9 Protein, (amino acids Ala20-Asp707) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-His-Avi
生物素化 no
确定序列 Ala20-Asp707
分子量 The recombinant Human MMP-9 protein with the His-Avi tag at the C-terminus has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human MMP-9 protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-MMP-9 Antibody protein with the C-hFc tag with the EC50 of 6.5 ng/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human MMP-9 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human MMP-9 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human MMP-9 Protein performance should be determined experimentally by the investigator.
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文献

BP005636: Human MMP-9 Protein with the C-His-Avi Tag (人 MMP-9 蛋白 c-his-avi 标签)

 

Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.

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