Human MICA Protein C-hFc Tag | Syd Labs BP003564

Human MICA Protein BP003564 Syd Labs

Human MICA Protein C-hFc Tag | Syd Labs BP003564

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(悉得)Syd Labs 货号:BP003564 Human MICA Protein with the C-hFc Tag (人 MICA 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu24-Gln308.

SKU: BP003564 分类: ,

产品参数

货号 BP003564
产品名称Human MICA Protein C-hFc Tag | Syd Labs BP003564
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
物种 Human
来源 The recombinant Human MICA Protein, (amino acids Glu24-Gln308) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-hFc
生物素化 no
确定序列 Glu24-Gln308
分子量 The recombinant Human MICA protein with the hFc tag at the C-terminus has a predicted MW of 59.64 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human NKG2D protein with the C-hFc tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human MICA protein with the C-hFc tag with the EC50 of 3.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.36 uM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human MICA Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human MICA Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Human MICA Protein performance should be determined experimentally by the investigator.
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文献

BP003564: Human MICA Protein with the C-hFc Tag (人 MICA 蛋白 C-hFc 标签)

 

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..

请记住我们的产品信息: 人 MICA 蛋白 (C-hFc 标签)BP003564 Syd Labs(Human MICA Protein with the C-hFc Tag).