Human Mature TGF beta 1 Protein No Tag | Syd Labs BP005661
(悉得）Syd Labs 货号：BP005661 Human Mature TGF beta 1 Protein with No Tag (人成熟 TGF β 1 蛋白 无标签) is expressed from HEK293 without tag.It contains Ala279-Ser390.
产品参数
| 货号 | BP005661 |
|---|---|
| 产品名称 | Human Mature TGF beta 1 Protein No Tag | Syd Labs BP005661 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1 |
| 物种 | Human |
| 来源 | The recombinant Human Mature TGF beta 1 Protein, (amino acids Ala279-Ser390) fused with No Tag, was produced in HEK 293 cells. |
| Accession | P01137 |
| 产品标签 | No Tag |
| 生物素化 | no |
| 确定序列 | Ala279-Ser390 |
| 分子量 | The recombinant Human Mature TGF beta 1 Protein has a predicted MW of 13.2 kDa (monomer). Due to glycosylation, the protein migrates to 14-15 kDa (monomer) under reduced (R) condition and 26-30 kDa (dimer) under Non reducing (N) condition based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Human Mature TGF beta 1, No tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TGF-beta RII, mFc tag with the EC50 of 3.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.35 uM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Mature TGF beta 1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Mature TGF beta 1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human Mature TGF beta 1 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005661: Human Mature TGF beta 1 Protein with No Tag (人成熟 TGF β 1 蛋白无标签)
TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration.
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