Human MASP2 Protein C-His Tag | Syd Labs BP005256
(悉得）Syd Labs 货号：BP005256 Human MASP2 Protein with the C-His Tag (人 MASP2 蛋白 C-His 标签) is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.
产品参数
| 货号 | BP005256 |
|---|---|
| 产品名称 | Human MASP2 Protein C-His Tag | Syd Labs BP005256 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | MAP19; MASP-2; MASP1P1; sMap |
| 物种 | Human |
| 来源 | The recombinant Human MASP2 Protein, (amino acids Gly288-Phe686) fused with a His tag at the C-terminus, was produced in E.coli cells. |
| Accession | O00187-1 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Gly288-Phe686 |
| 分子量 | The recombinant Human MASP2 protein with the His tag at the C-terminus has a predicted MW of 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human MASP2 protein with the C-His tag at 0.5 ug/ml (100 ul/Well). Dose response curve for the recombinant Anti-MASP2 Antibody protein with the C-hFc tag with the EC50 of 5.0 ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human MASP2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human MASP2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human MASP2 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005256: Human MASP2 Protein with the C-His Tag (人 MASP2 蛋白 C-His 标签)
The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.
请记住我们的产品信息: 人 MASP2 蛋白 （C-His 标签）BP005256 Syd Labs（Human MASP2 Protein with the C-His Tag）.
