Human LRP-5 Protein BP004356 Syd Labs
Human LRP-5 Protein (LRP-HM305)
Human LRP-5 Protein BP004356 Syd Labs
Human LRP-5 Protein (LRP-HM305)

Human LRP-5 Protein C-mFc Tag | Syd Labs BP004356

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Human LRP-5 Protein BP004356 Syd Labs

Human LRP-5 Protein C-mFc Tag | Syd Labs BP004356

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(悉得）Syd Labs 货号：BP004356 Human LRP-5 Protein with the C-mFc Tag (人 LRP-5 蛋白 C-mFc 标签) is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Glu644-Gln1263.

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SKU： BP004356 分类： ,

产品参数

货号 BP004356
产品名称Human LRP-5 Protein C-mFc Tag | Syd Labs BP004356
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2
物种 Human
来源 The recombinant Human LRP-5 Protein, (amino acids Glu644-Gln1263) fused with a mFc tag at the C-terminus, was produced in HEK 293 cells.
Accession O75197-1
产品标签 C-mFc
生物素化 no
确定序列 Glu644-Gln1263
分子量 The recombinant Human LRP-5 protein with the mFc tag at the C-terminus has a predicted MW of 96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human DKK1 protein with the C-mFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human LRP-5 protein with the C-mFc tag with the EC50 of 0.11 ug/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human LRP-5 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human LRP-5 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Human LRP-5 Protein performance should be determined experimentally by the investigator.
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文献

BP004356: Human LRP-5 Protein with the C-mFc Tag (人 LRP-5 蛋白 C-mFc 标签)

 

A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.

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