Human LILRB4/CD85k/ILT3 Protein C-His-Avi Tag | Syd Labs BP005510
(悉得）Syd Labs 货号：BP005510 Human LILRB4/CD85k/ILT3 Protein with the C-His-Avi Tag (人 LILRB4/CD85k/ILT3 蛋白 c-his-avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His257.
产品参数
| 货号 | BP005510 |
|---|---|
| 产品名称 | Human LILRB4/CD85k/ILT3 Protein C-His-Avi Tag | Syd Labs BP005510 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K |
| 物种 | Human |
| 来源 | The recombinant Human LILRB4/CD85k/ILT3 Protein, (amino acids Gln22-His257) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Gln22-His257 |
| 分子量 | The recombinant Human LILRB4/CD85k/ILT3 protein with the His-Avi tag at the C-terminus has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human LILRB4 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-LILRB4 Antibody protein with the C-hFc tag with the EC50 of 9.5 ng/ml determined by ELISA (QC Test). The affinity constant of 11.87 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human LILRB4/CD85k/ILT3 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human LILRB4/CD85k/ILT3 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human LILRB4/CD85k/ILT3 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005510: Human LILRB4/CD85k/ILT3 Protein with the C-His-Avi Tag (人 LILRB4/CD85k/ILT3 蛋白 c-his-avi 标签)
LILRB4,also known as CD85k and LIR-5, ILT-3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
请记住我们的产品信息: 人 LILRB4/CD85k/ILT3 蛋白 （c-his-avi 标签）BP005510 Syd Labs（Human LILRB4/CD85k/ILT3 Protein with the C-His-Avi Tag）.