Human LILRB2/CD85d/ILT4 Protein C-hFc Tag | Syd Labs BP004941
(悉得）Syd Labs 货号：BP004941 Human LILRB2/CD85d/ILT4 Protein with the C-hFc Tag (人 LILRB2/CD85d/ILT4 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-His458.
产品参数
| 货号 | BP004941 |
|---|---|
| 产品名称 | Human LILRB2/CD85d/ILT4 Protein C-hFc Tag | Syd Labs BP004941 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10 |
| 物种 | Human |
| 来源 | The recombinant Human LILRB2/CD85d/ILT4 Protein, (amino acids Gln22-His458) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Gln22-His458 |
| 分子量 | The recombinant Human LILRB2/CD85d/ILT4 protein with the hFc tag at the C-terminus has a predicted MW of 74.1 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human LILRB2 protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-LILRB2 Antibody protein with the C-hFc tag with the EC50 of 14.6 ng/ml determined by ELISA (QC Test). The affinity constant of 9.20 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human LILRB2/CD85d/ILT4 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human LILRB2/CD85d/ILT4 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human LILRB2/CD85d/ILT4 Protein performance should be determined experimentally by the investigator. |
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文献
BP004941: Human LILRB2/CD85d/ILT4 Protein with the C-hFc Tag (人 LILRB2/CD85d/ILT4 蛋白 C-hFc 标签)
The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
请记住我们的产品信息: 人 LILRB2/CD85d/ILT4 蛋白 （C-hFc 标签）BP004941 Syd Labs（Human LILRB2/CD85d/ILT4 Protein with the C-hFc Tag）.