Human IL-21R Protein C-hFc Tag | Syd Labs BP005050

Human IL-21R Protein BP005050 Syd Labs

Human IL-21R Protein C-hFc Tag | Syd Labs BP005050

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(悉得)Syd Labs 货号:BP005050 Human IL-21R Protein with the C-hFc Tag (人 IL-21R 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys20-Pro236.

SKU: BP005050 分类: ,

产品参数

货号 BP005050
产品名称Human IL-21R Protein C-hFc Tag | Syd Labs BP005050
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 IL-21 receptor; IL-21R; CD360; NILR
物种 Human
来源 The recombinant Human IL-21R Protein, (amino acids Cys20-Pro236) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Accession NP_068570.1
产品标签 C-hFc
生物素化 no
确定序列 Cys20-Pro236
分子量 The recombinant Human IL-21R protein with the hFc tag at the C-terminus has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human IL-21 protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IL-21R protein with the C-hFc tag with the EC50 of 42.3 ng/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human IL-21R Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human IL-21R Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human IL-21R Protein performance should be determined experimentally by the investigator.
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文献

BP005050: Human IL-21R Protein with the C-hFc Tag (人 IL-21R 蛋白 C-hFc 标签)

 

IL-21 and IL-21R were highly expressed in the lesional skin and peripheral blood of psoriasis patients. IL-21 promoted CD4 T cells proliferation and Th17 cells differentiation and inhibiting Treg cells differentiation by upregulating RORγt expression and downregulating Foxp3 expression, with increased expression and secretion of IL-17A and IL-22.Microbial translocation and the associated immune activation during HIV-1 infection may lead to high expression levels of the IL-21R activation marker in RM B cells, a feature associated with increased apoptosis and a reduced number of these cells in the circulation.

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