Human IL-21R Protein C-hFc Tag | Syd Labs BP005050
(悉得)Syd Labs 货号:BP005050 Human IL-21R Protein with the C-hFc Tag (人 IL-21R 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys20-Pro236.
产品参数
| 货号 | BP005050 |
|---|---|
| 产品名称 | Human IL-21R Protein C-hFc Tag | Syd Labs BP005050 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | IL-21 receptor; IL-21R; CD360; NILR |
| 物种 | Human |
| 来源 | The recombinant Human IL-21R Protein, (amino acids Cys20-Pro236) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | NP_068570.1 |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Cys20-Pro236 |
| 分子量 | The recombinant Human IL-21R protein with the hFc tag at the C-terminus has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human IL-21 protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IL-21R protein with the C-hFc tag with the EC50 of 42.3 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IL-21R Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human IL-21R Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human IL-21R Protein performance should be determined experimentally by the investigator. |
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文献
BP005050: Human IL-21R Protein with the C-hFc Tag (人 IL-21R 蛋白 C-hFc 标签)
IL-21 and IL-21R were highly expressed in the lesional skin and peripheral blood of psoriasis patients. IL-21 promoted CD4 T cells proliferation and Th17 cells differentiation and inhibiting Treg cells differentiation by upregulating RORγt expression and downregulating Foxp3 expression, with increased expression and secretion of IL-17A and IL-22.Microbial translocation and the associated immune activation during HIV-1 infection may lead to high expression levels of the IL-21R activation marker in RM B cells, a feature associated with increased apoptosis and a reduced number of these cells in the circulation.
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