Human IL-1R1 Protein C-His Tag | Syd Labs BP004482
Syd Labs 货号：BP004482 Human IL-1R1 Protein with the C-His Tag (人 IL-1R1 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Thr332.
产品参数
| 货号 | BP004482 |
|---|---|
| 产品名称 | Human IL-1R1 Protein C-His Tag | Syd Labs BP004482 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | IL-1R-1; IL-1RT1; IL-1R-alpha; CD121a; IL-1 RI; IL1R; IL1RI; IL-1RI; p80; D2S1473; IL1RA |
| 物种 | Human |
| 来源 | The recombinant Human IL-1R1 Protein, (amino acids Leu18-Thr332) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Leu18-Thr332 |
| 分子量 | The recombinant Human IL-1R1 protein with the His tag at the C-terminus has a predicted MW of 37 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human IL-1R1 protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-1 Beta protein with the C-His tag with the EC50 of 0.13 ug/ml determined by ELISA (QC Test). The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IL-1R1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human IL-1R1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human IL-1R1 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004482: Human IL-1R1 Protein with the C-His Tag (人 IL-1R1 蛋白 C-His 标签)
Interleukin 1 (IL-1) has long been known for its pleiotropic effects on inflammation that plays a complex, and sometimes contrasting, role in different stages of cancer development.IL-1R1 is the main receptor for both ligands and is expressed by various cell types, including innate and adaptive immune cell types, epithelial cells, endothelial cells, adipocytes, chondrocytes, fibroblasts, etc. IL-1 and IL-1R1 receptor interaction leads to a set of common signaling pathways, mainly the NF-kB and MAP kinase pathways, as a result of complex positive and negative regulations.
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