Human IL-13 Protein N-His-Avi Tag | Syd Labs BP005417

Human IL-13 Protein BP005417 Syd Labs

Human IL-13 Protein N-His-Avi Tag | Syd Labs BP005417

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(悉得)Syd Labs 货号:BP005417 Human IL-13 Protein with the N-His-Avi Tag (人 IL-13 蛋白 N-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gly21-Asn132.

SKU: BP005417 分类: ,

产品参数

货号 BP005417
产品名称Human IL-13 Protein N-His-Avi Tag | Syd Labs BP005417
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
物种 Human
来源 The recombinant Human IL-13 Protein, (amino acids Gly21-Asn132) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Accession AAK53823
产品标签 N-His-Avi
生物素化 no
确定序列 Gly21-Asn132
分子量 The recombinant Human IL-13 protein with the His-Avi tag at the N-terminus has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 25-45 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human IL-13 protein with the N-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IL-13Ra2 protein with the N-hFc tag with the EC50 of 1.0 ug/ml determined by ELISA (QC Test). The affinity constant of 1 uM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human IL-13 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human IL-13 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human IL-13 Protein performance should be determined experimentally by the investigator.
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文献

BP005417: Human IL-13 Protein with the N-His-Avi Tag (人 IL-13 蛋白 N-His-Avi 标签)

 

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

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