Human IL-12 Protein C-His-Avi Tag | Syd Labs BP005622
(悉得）Syd Labs 货号：BP005622 Human IL-12 Protein with the C-His-Avi Tag (人 IL-12 蛋白 c-his-avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile23-Ser328 (IL-12B) & Arg23-Ser219 (IL-12A).
产品参数
| 货号 | BP005622 |
|---|---|
| 产品名称 | Human IL-12 Protein C-His-Avi Tag | Syd Labs BP005622 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12 |
| 物种 | Human |
| 来源 | The recombinant Human IL-12 Protein, (amino acids Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P29460(IL-12B)&P29459(IL-12A) |
| 产品标签 | C-His-Avi |
| 生物素化 | no |
| 确定序列 | Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A) |
| 分子量 | The recombinant Human IL-12 protein with the His-Avi tag at the C-terminus has a predicted MW of 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human IL-12 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-IL-12 Antibody protein with the C-hFc tag with the EC50 of 2.1 ng/ml determined by ELISA (QC Test). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IL-12 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human IL-12 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human IL-12 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005622: Human IL-12 Protein with the C-His-Avi Tag (人 IL-12 蛋白 c-his-avi 标签)
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
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