Human IL-10 Protein N-His-Avi Tag | Syd Labs BP005589
(悉得）Syd Labs 货号：BP005589 Human IL-10 Protein with the N-His-Avi Tag (人 IL-10 蛋白 N-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ser19-Asn178.
产品参数
| 货号 | BP005589 |
|---|---|
| 产品名称 | Human IL-10 Protein N-His-Avi Tag | Syd Labs BP005589 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451 |
| 物种 | Human |
| 来源 | The recombinant Human IL-10 Protein, (amino acids Ser19-Asn178) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells. |
| 产品标签 | N-His-Avi |
| 生物素化 | no |
| 确定序列 | Ser19-Asn178 |
| 分子量 | The recombinant Human IL-10 protein with the His-Avi tag at the N-terminus has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human IL-10 protein with the N-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-10R alpha protein with the N-His tag with the EC50 of 28.7 ng/ml determined by ELISA (QC Test). The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IL-10 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human IL-10 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human IL-10 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005589: Human IL-10 Protein with the N-His-Avi Tag (人 IL-10 蛋白 N-His-Avi 标签)
Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
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