Human IGFI Protein BP003423 Syd Labs
Human IGFI Protein (IGF-HM201)
Human IGFI Protein (IGF-HM201)
Human IGFI Protein (IGF-HM201)
Human IGFI Protein BP003423 Syd Labs
Human IGFI Protein (IGF-HM201)
Human IGFI Protein (IGF-HM201)
Human IGFI Protein (IGF-HM201)

Human IGFI Protein N-hFc Tag | Syd Labs BP003423

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Human IGFI Protein BP003423 Syd Labs

Human IGFI Protein N-hFc Tag | Syd Labs BP003423

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(悉得）Syd Labs 货号：BP003423 Human IGFI Protein with the N-hFc Tag (人 IGFI 蛋白 N-hFc 标签) is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gly49-Ala118.

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Syd Labs, Inc.
SKU： BP003423 分类： ,

产品参数

货号 BP003423
产品名称Human IGFI Protein N-hFc Tag | Syd Labs BP003423
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
物种 Human
来源 The recombinant Human IGFI Protein, (amino acids Gly49-Ala118) fused with a hFc tag at the N-terminus, was produced in HEK 293 cells.
产品标签 N-hFc
生物素化 no
确定序列 Gly49-Ala118
分子量 The recombinant Human IGFI protein with the hFc tag at the N-terminus has a predicted MW of 33.18 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human IGF1R protein with the N-hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IGFI protein with the N-hFc tag with the EC50 of 1.25 ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human IGFI Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human IGFI Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Human IGFI Protein performance should be determined experimentally by the investigator.
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文献

BP003423: Human IGFI Protein with the N-hFc Tag (人 IGFI 蛋白 N-hFc 标签)

 

The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes.

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