Human IGFBP-3 Protein C-His Tag | Syd Labs BP004780
Syd Labs 货号：BP004780 Human IGFBP-3 Protein with the C-His Tag (人 IGFBP-3 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Gly28-Lys291.
产品参数
| 货号 | BP004780 |
|---|---|
| 产品名称 | Human IGFBP-3 Protein C-His Tag | Syd Labs BP004780 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | IBP3; IGFBP3; IGFBP-3; BP-53 |
| 物种 | Human |
| 来源 | The recombinant Human IGFBP-3 Protein, (amino acids Gly28-Lys291) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Gly28-Lys291 |
| 分子量 | The recombinant Human IGFBP-3 protein with the His tag at the C-terminus has a predicted MW of 29.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human IGFBP-3 protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IGF-II protein with the C-hFc tag with the EC50 of 0.19 ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human IGFBP-3 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human IGFBP-3 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human IGFBP-3 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004780: Human IGFBP-3 Protein with the C-His Tag (人 IGFBP-3 蛋白 C-His 标签)
Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer.
请记住我们的产品信息: 人 IGFBP-3 蛋白 （C-His 标签）BP004780 Syd Labs（Human IGFBP-3 Protein with the C-His Tag）.