Human HMGB1 Protein C-His Tag | Syd Labs BP005293
(悉得）Syd Labs 货号：BP005293 Human HMGB1 Protein with the C-His Tag (人 HMGB1 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Met1-Glu215.
产品参数
| 货号 | BP005293 |
|---|---|
| 产品名称 | Human HMGB1 Protein C-His Tag | Syd Labs BP005293 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | HMGB1; HMG1; HMG3; SBP-1; HMG-1 |
| 物种 | Human |
| 来源 | The recombinant Human HMGB1 Protein, (amino acids Met1-Glu215) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P09429-1 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Met1-Glu215 |
| 分子量 | The recombinant Human HMGB1 protein with the His tag at the C-terminus has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human HMGB1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human HMGB1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human HMGB1 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005293: Human HMGB1 Protein with the C-His Tag (人 HMGB1 蛋白 C-His 标签)
High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation.
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