Human GFRAL/GFR alpha-like Protein BP005207 Syd Labs
Human GFRAL/GFR alpha-like Protein (GFL-HM201)
Human GFRAL/GFR alpha-like Protein (GFL-HM201)
Human GFRAL/GFR alpha-like Protein (GFL-HM201)
Human GFRAL/GFR alpha-like Protein BP005207 Syd Labs
Human GFRAL/GFR alpha-like Protein (GFL-HM201)
Human GFRAL/GFR alpha-like Protein (GFL-HM201)
Human GFRAL/GFR alpha-like Protein (GFL-HM201)

Human GFRAL/GFR alpha-like Protein C-hFc Tag | Syd Labs BP005207

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Human GFRAL/GFR alpha-like Protein BP005207 Syd Labs

Human GFRAL/GFR alpha-like Protein C-hFc Tag | Syd Labs BP005207

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(悉得）Syd Labs 货号：BP005207 Human GFRAL/GFR alpha-like Protein with the C-hFc Tag (人 GFRAL/GFR α 样蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser19-Glu351.

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Syd Labs, Inc.
SKU： BP005207 分类： ,

产品参数

货号 BP005207
产品名称Human GFRAL/GFR alpha-like Protein C-hFc Tag | Syd Labs BP005207
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 GFR alpha-like; GFRAL; GRAL; C6orf144
物种 Human
来源 The recombinant Human GFRAL/GFR alpha-like Protein, (amino acids Ser19-Glu351) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-hFc
生物素化 no
确定序列 Ser19-Glu351
分子量 The recombinant Human GFRAL/GFR alpha-like protein with the hFc tag at the C-terminus has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human GDF15 protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human GFRAL protein with the C-hFc tag with the EC50 of 11.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human GFRAL/GFR alpha-like Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human GFRAL/GFR alpha-like Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human GFRAL/GFR alpha-like Protein performance should be determined experimentally by the investigator.
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文献

BP005207: Human GFRAL/GFR alpha-like Protein with the C-hFc Tag (人 GFRAL/GFR α 样蛋白 C-hFc 标签)

 

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

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