Human GDF15 Protein N-His Tag | Syd Labs BP004307
Syd Labs 货号：BP004307 Human GDF15 Protein with the N-His Tag (人 GDF15 蛋白 N-His 标签) is expressed from E.coli with His tag at the N-Terminus.It contains Ala197-Ile308.
产品参数
| 货号 | BP004307 |
|---|---|
| 产品名称 | Human GDF15 Protein N-His Tag | Syd Labs BP004307 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta |
| 物种 | Human |
| 来源 | The recombinant Human GDF15 Protein, (amino acids Ala197-Ile308) fused with a His tag at the N-terminus, was produced in E.coli cells. |
| 产品标签 | N-His |
| 生物素化 | no |
| 确定序列 | Ala197-Ile308 |
| 分子量 | The recombinant Human GDF15 protein with the His tag at the N-terminus has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human GDF15 protein with the N-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human GFRAL protein with the N-hFc tag with the EC50 of 11.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human GDF15 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human GDF15 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human GDF15 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004307: Human GDF15 Protein with the N-His Tag (人 GDF15 蛋白 N-His 标签)
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
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