Human Fc epsilon RI alpha/FCER1a Protein C-His Tag | Syd Labs BP004330
(悉得）Syd Labs 货号：BP004330 Human Fc epsilon RI alpha/FCER1a Protein with the C-His Tag (人 Fc ε RI α/FCER1a 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Val26-Gln205.
产品参数
| 货号 | BP004330 |
|---|---|
| 产品名称 | Human Fc epsilon RI alpha/FCER1a Protein C-His Tag | Syd Labs BP004330 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A |
| 物种 | Human |
| 来源 | The recombinant Human Fc epsilon RI alpha/FCER1a Protein, (amino acids Val26-Gln205) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P12319-1 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Val26-Gln205 |
| 分子量 | The recombinant Human Fc epsilon RI alpha/FCER1a protein with the His tag at the C-terminus has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human Fc epsilon RI alpha protein with the C-His tag at 0.2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Fc epsilon RI alpha Antibody protein with the C-hFc tag with the EC50 of 4.0 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Fc epsilon RI alpha/FCER1a Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1EU per ug by the LAL method. |
| 运输 | The recombinant Human Fc epsilon RI alpha/FCER1a Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human Fc epsilon RI alpha/FCER1a Protein performance should be determined experimentally by the investigator. |
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文献
BP004330: Human Fc epsilon RI alpha/FCER1a Protein with the C-His Tag (人 Fc ε RI α/FCER1a 蛋白 C-His 标签)
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
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