Human Ephrin-B2/EFNB2 Protein C-hFc Tag | Syd Labs BP005087
(悉得）Syd Labs 货号：BP005087 Human Ephrin-B2/EFNB2 Protein with the C-hFc Tag (人 Ephrin-B2/EFNB2 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile28-Ala229.
产品参数
| 货号 | BP005087 |
|---|---|
| 产品名称 | Human Ephrin-B2/EFNB2 Protein C-hFc Tag | Syd Labs BP005087 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | EPLG5; HTKL; LERK5; EFNB2 |
| 物种 | Human |
| 来源 | The recombinant Human Ephrin-B2/EFNB2 Protein, (amino acids Ile28-Ala229) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | NP_004084.1 |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Ile28-Ala229 |
| 分子量 | The recombinant Human Ephrin-B2/EFNB2 protein with the hFc tag at the C-terminus has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human EPHB2 protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human Ephrin-B2 protein with the C-hFc tag with the EC50 of 3.2 ng/ml determined by ELISA (QC Test). The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human Ephrin-B2/EFNB2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human Ephrin-B2/EFNB2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human Ephrin-B2/EFNB2 Protein performance should be determined experimentally by the investigator. |
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文献
BP005087: Human Ephrin-B2/EFNB2 Protein with the C-hFc Tag (人 Ephrin-B2/EFNB2 蛋白 C-hFc 标签)
Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.
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