Human EPHB2 Protein BP004794 Syd Labs
Human EPHB2 Protein (EPH-HM101)
Human EPHB2 Protein (EPH-HM101)
Human EPHB2 Protein BP004794 Syd Labs
Human EPHB2 Protein (EPH-HM101)
Human EPHB2 Protein (EPH-HM101)

Human EPHB2 Protein C-His Tag | Syd Labs BP004794

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Human EPHB2 Protein BP004794 Syd Labs

Human EPHB2 Protein C-His Tag | Syd Labs BP004794

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(悉得）Syd Labs 货号：BP004794 Human EPHB2 Protein with the C-His Tag (人 EPHB2 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Leu543.

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SKU： BP004794 分类： ,

产品参数

货号 BP004794
产品名称Human EPHB2 Protein C-His Tag | Syd Labs BP004794
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 DRT; ERK; HEK5; TYRO5; CAPB; EK5; EPHT3; PCBC; Cek5; EphB2; EPHT3MGC87492; Nuk; Qek2; Sek3
物种 Human
来源 The recombinant Human EPHB2 Protein, (amino acids Val19-Leu543) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
产品标签 C-His
生物素化 no
确定序列 Val19-Leu543
分子量 The recombinant Human EPHB2 protein with the His tag at the C-terminus has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human EPHB2 protein with the C-His tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-EPHB2 Antibody protein with the C-hFc tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test). The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human EPHB2 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human EPHB2 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human EPHB2 Protein performance should be determined experimentally by the investigator.
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文献

BP004794: Human EPHB2 Protein with the C-His Tag (人 EPHB2 蛋白 C-His 标签)

 

EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.

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