Human EPHA3 Protein C-His Tag | Syd Labs BP004406
(悉得）Syd Labs 货号：BP004406 Human EPHA3 Protein with the C-His Tag (人 EPHA3 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Gln541.
产品参数
| 货号 | BP004406 |
|---|---|
| 产品名称 | Human EPHA3 Protein C-His Tag | Syd Labs BP004406 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | EK4; HEK; ETK; ETK1; EPHA3; TYRO4; HEK4 |
| 物种 | Human |
| 来源 | The recombinant Human EPHA3 Protein, (amino acids Glu21-Gln541) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | P29320-1 |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Glu21-Gln541 |
| 分子量 | The recombinant Human EPHA3 protein with the His tag at the C-terminus has a predicted MW of 59.9 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human EPHA3 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human EPHA3 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution. |
| 注意事项 | Conditions of optimal recombinant Human EPHA3 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP004406: Human EPHA3 Protein with the C-His Tag (人 EPHA3 蛋白 C-His 标签)
Erythropoietin‑producing hepatocellular carcinoma cell surface type‑A receptor 3 (EPHA3) has been found to promote the proliferation and survival of prostate cancer (PCa) cell lines and prostate tumor development in nude mice. The interaction of AR and SP1 contributes to regulate EPHA3 expression, and the SP1 binding sites (‑295~‑261) in the EPHA3 core promoter region is crucial to the regulation of EPHA3 expression in response to androgen hormone stimuli.
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