Human EGF Protein No Tag | Syd Labs BP005758

Human EGF Protein BP005758 Syd Labs

Human EGF Protein No Tag | Syd Labs BP005758

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(悉得)Syd Labs 货号:BP005758 Human EGF Protein with No Tag (人 EGF 蛋白 No Tag) is expressed from E.coli without tag.It contains Asn971-Arg1023.

SKU: BP005758 分类: ,

产品参数

货号 BP005758
产品名称Human EGF Protein No Tag | Syd Labs BP005758
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 HOMG4; EGF; URG
物种 Human
来源 The recombinant Human EGF Protein, (amino acids Asn971-Arg1023) fused with No Tag, was produced in E.coli cells.
Accession P01133-1
产品标签 No Tag
生物素化 no
确定序列 Asn971-Arg1023
分子量 The recombinant Human EGF Protein has a predicted MW of 5.6 kDa same as Tris-Bis PAGE result.
活性 Immobilized recombinant Human EGF, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human EGFR, hFc tag with the EC50 of 0.82 ug/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human EGF Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human EGF Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human EGF Protein performance should be determined experimentally by the investigator.
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文献

BP005758: Human EGF Protein with No Tag (人 EGF 蛋白 No Tag)

 

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

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