Human EGF Protein No Tag | Syd Labs BP005758
(悉得)Syd Labs 货号:BP005758 Human EGF Protein with No Tag (人 EGF 蛋白 No Tag) is expressed from E.coli without tag.It contains Asn971-Arg1023.
产品参数
| 货号 | BP005758 |
|---|---|
| 产品名称 | Human EGF Protein No Tag | Syd Labs BP005758 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | HOMG4; EGF; URG |
| 物种 | Human |
| 来源 | The recombinant Human EGF Protein, (amino acids Asn971-Arg1023) fused with No Tag, was produced in E.coli cells. |
| Accession | P01133-1 |
| 产品标签 | No Tag |
| 生物素化 | no |
| 确定序列 | Asn971-Arg1023 |
| 分子量 | The recombinant Human EGF Protein has a predicted MW of 5.6 kDa same as Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human EGF, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human EGFR, hFc tag with the EC50 of 0.82 ug/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human EGF Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human EGF Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human EGF Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品,不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题,欢迎随时联系Syd Labs国内市场推广合作伙伴:武汉多找找科技有限公司,企业微信:duozhaozhao2024 联系电话:18162581039(龙经理) |
文献
BP005758: Human EGF Protein with No Tag (人 EGF 蛋白 No Tag)
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
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