Human CD73/NT5E Protein BP005073 Syd Labs
Human CD73/NT5E Protein (CD7-HM273)
Human CD73/NT5E Protein (CD7-HM273)
Human CD73/NT5E Protein BP005073 Syd Labs
Human CD73/NT5E Protein (CD7-HM273)
Human CD73/NT5E Protein (CD7-HM273)

Human CD73/NT5E Protein C-hFc Tag | Syd Labs BP005073

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Human CD73/NT5E Protein BP005073 Syd Labs

Human CD73/NT5E Protein C-hFc Tag | Syd Labs BP005073

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(悉得）Syd Labs 货号：BP005073 Human CD73/NT5E Protein with the C-hFc Tag (人 CD73/NT5E 蛋白 C-hFc 标签) is expressed from HEK293 with hFC tag at the C-Terminus.It contains Trp27-Lys547.

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SKU： BP005073 分类： ,

产品参数

货号 BP005073
产品名称Human CD73/NT5E Protein C-hFc Tag | Syd Labs BP005073
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
物种 Human
来源 The recombinant Human CD73/NT5E Protein, (amino acids Trp27-Lys547) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Accession P21589-1
产品标签 C-hFc
生物素化 no
确定序列 Trp27-Lys547
分子量 The recombinant Human CD73/NT5E protein with the hFc tag at the C-terminus has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human CD73 protein with the C-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-CD73 Antibody protein with the C-hFc tag with the EC50 of 22.6 ng/ml determined by ELISA (QC Test).
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human CD73/NT5E Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human CD73/NT5E Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human CD73/NT5E Protein performance should be determined experimentally by the investigator.
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文献

BP005073: Human CD73/NT5E Protein with the C-hFc Tag (人 CD73/NT5E 蛋白 C-hFc 标签)

 

CD73, also known as ecto-5′-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A  deficiency of CD73 occurs in a variety of immunodeficiency diseases.

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