Human CD3E/CD3 epsilon Protein C-hFc Tag | Syd Labs BP005441
Syd Labs 货号：BP005441 Human CD3E/CD3 epsilon Protein with the C-hFc Tag (人 CD3E/CD3 ε 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp23-Asp126(C119S, C122S).
产品参数
| 货号 | BP005441 |
|---|---|
| 产品名称 | Human CD3E/CD3 epsilon Protein C-hFc Tag | Syd Labs BP005441 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
| 物种 | Human |
| 来源 | The recombinant Human CD3E/CD3 epsilon Protein, (amino acids Asp23-Asp126(C119S, C122S)) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Asp23-Asp126(C119S, C122S) |
| 分子量 | The recombinant Human CD3E/CD3 epsilon protein with the hFc tag at the C-terminus has a predicted MW of 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human CD3E protein with the C-hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Anti-CD3 Antibody protein with the C-hFc tag with the EC50 of 33.5 ng/ml determined by ELISA (QC Test). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human CD3E/CD3 epsilon Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human CD3E/CD3 epsilon Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human CD3E/CD3 epsilon Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005441: Human CD3E/CD3 epsilon Protein with the C-hFc Tag (人 CD3E/CD3 ε 蛋白 C-hFc 标签)
T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
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