Human CD19 Protein C-His Tag | Syd Labs BP005439
(悉得）Syd Labs 货号：BP005439 Human CD19 Protein with the C-His Tag (人 CD19 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Pro20-Lys291.
产品参数
| 货号 | BP005439 |
|---|---|
| 产品名称 | Human CD19 Protein C-His Tag | Syd Labs BP005439 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD19; B4; CVID3; Leu-12; MGC12802 |
| 物种 | Human |
| 来源 | The recombinant Human CD19 Protein, (amino acids Pro20-Lys291) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Pro20-Lys291 |
| 分子量 | The recombinant Human CD19 protein with the His tag at the C-terminus has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result. |
| 活性 | Immobilized recombinant Human CD19 protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Coltuximab protein with the C-hFc tag with the EC50 of 11.3 ng/ml determined by ELISA (QC Test). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human CD19 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human CD19 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human CD19 Protein performance should be determined experimentally by the investigator. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
BP005439: Human CD19 Protein with the C-His Tag (人 CD19 蛋白 C-His 标签)
CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses.Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
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