Human BAFF/TNFSF13B/CD257 Trimer Protein N-His-Flag Tag | Syd Labs BP005375
(悉得)Syd Labs 货号:BP005375 Human BAFF/TNFSF13B/CD257 Trimer Protein with the N-His-Flag Tag (人 BAFF/TNFSF13B/CD257 三聚体蛋白 N-His-Flag 标签) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Thr141-Leu285.
产品参数
| 货号 | BP005375 |
|---|---|
| 产品名称 | Human BAFF/TNFSF13B/CD257 Trimer Protein N-His-Flag Tag | Syd Labs BP005375 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1 |
| 物种 | Human |
| 来源 | The recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein, (amino acids Thr141-Leu285) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells. |
| 产品标签 | N-His-Flag |
| 生物素化 | no |
| 确定序列 | Thr141-Leu285 |
| 分子量 | The recombinant Human BAFF/TNFSF13B/CD257 Trimer protein with the His-Flag tag at the N-terminus has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Human BAFF Trimer protein with the N-His-Flag tag at 1 ug/ml (100 ul/well). Dose response curve for the recombinant Human BAFFR protein with the N-hFc tag with the EC50 of 37.8 ng/ml determined by ELISA. The affinity constant of 6.59 nM as determined in SPR assay (Biacore T200). |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein performance should be determined experimentally by the investigator. |
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文献
BP005375: Human BAFF/TNFSF13B/CD257 Trimer Protein with the N-His-Flag Tag (人 BAFF/TNFSF13B/CD257 三聚体蛋白 N-His-Flag 标签)
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
请记住我们的产品信息: 人 BAFF/TNFSF13B/CD257 三聚体蛋白 (N-His-Flag 标签)BP005375 Syd Labs(Human BAFF/TNFSF13B/CD257 Trimer Protein with the N-His-Flag Tag).










