Human APLN Protein C-hFc Tag | Syd Labs BP004914

Human APLN Protein BP004914 Syd Labs

Human APLN Protein C-hFc Tag | Syd Labs BP004914

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(悉得)Syd Labs 货号:BP004914 Human APLN Protein with the C-hFc Tag (人 APLN 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly23-Phe77.

SKU: BP004914 分类: ,

产品参数

货号 BP004914
产品名称Human APLN Protein C-hFc Tag | Syd Labs BP004914
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
别称 Apelin;APJ endogenous ligand;APEL
物种 Human
来源 The recombinant Human APLN Protein, (amino acids Gly23-Phe77) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Accession Q9ULZ1
产品标签 C-hFc
生物素化 no
确定序列 Gly23-Phe77
分子量 The recombinant Human APLN protein with the hFc tag at the C-terminus has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human APLN protein with the C-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Anti-APLN Antibody protein with the C-hFc tag with the EC50 of 22.2 ng/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human APLN Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human APLN Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Human APLN Protein performance should be determined experimentally by the investigator.
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文献

BP004914: Human APLN Protein with the C-hFc Tag (人 APLN 蛋白 C-hFc 标签)

 

Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages. Additionally, due to the widespread distribution of the CVD, more effective therapeutic strategies need to be developed to overcome the related complications.

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