D-luciferin Syd Labs文献引用，总有一个是你需要的！

D-luciferin Syd Labs MB000102-R70170：武汉多找找科技合作进口D-luciferin品牌产品，Syd Labs D-luciferin d荧光素钾盐获得全球客户的肯定和信赖。D-luciferin Syd Labs产品被国内外很多文章引用，应用于体内活体成像（In vivo imaging）、体外研究（荧光素酶和ATP水平分析、报告基因分析、高通量测序和各种污染检测）。

D-luciferin Syd Labs具体应用文献：

3.

Phosphatidylserine receptors enhance SARS-CoV-2 infection

Dana Bohan, Hanora Van Ert, Natalie Ruggio, Kai J Rogers, Mohammad Badreddine, José A Aguilar Briseño, Jonah M Elliff, Roberth Anthony Rojas Chavez, Boning Gao, Tomasz Stokowy, Eleni Christakou, Petri Kursula, David Micklem, Gro Gausdal, Hillel Haim, John Minna, James B Lorens, Wendy Maury

PLoS Pathog.2021 Nov 19;17(11):e1009743. doi: 10.1371/journal.ppat.1009743. eCollection 2021 Nov.

Following the transfection of HEK 293T cells, cells were incubated for 24 hours. At that time cells were lifted with 0.25% Trypsin (GIBCO; 25200–056) and plated at a density of 2 X 104 cells/well on opaque, flat-bottomed, 96-well plates (Falcon; 353296). Each transfection was plated into at least 3 wells to create experimental replicates. Cells were incubated for an additional 24 hours. At that time, cells were infected with VSV-luciferase/SARS-CoV-2 spike. Cells were incubated for an additional 24 hours. For experiments done with inhibitors, cells were treated with the concentrations of inhibitors noted in the figure panel immediately prior to being infected with pseudotyped virions. After 24 h, virus-containing media was removed and replaced with 35 μL of 1x Passive lysis buffer (Promega; E194A). Plates underwent three freeze-thaw cycles consisting of freezing on dry-ice for 15 minutes followed by thawing at 37°C for 15 minutes. We followed the protocol for measuring firefly luciferase as reported previously (Johnson et al., 2017). For this method 100 μL of luciferin buffer (100 μl of luciferin buffer (15 mM MgSO4, 15mM KPO4 pH 7.8, 1 mM ATP, and 1mM dithiothreitol) and 50 μL of 1mM d-luciferin potassium salt (Syd Laboratories; MB000102-R70170)) were added to each well and luminescence was read via Synergy H1 Hybrid reader (BioTek Instruments). Relative luminescence units were read out. Results were analyzed by normalizing values to mock transfection with no protease inhibitors.

4.

Interleukin-17 promotes metastasis in an immunocompetent orthotopic mouse model of prostate cancer

David Cunningham, Qiuyang Zhang, Sen Liu, Keshab R Parajuli, Qiang Nie, Lin Ma, Allen Zhang, Zhenbang Chen, Zongbing You

Am J Clin Exp Urol. 2018 Jun 15;6(3):114-122. eCollection 2018.

Starting one-week post-surgery, primary tumor growth was assessed using IVIS Lumina XRMS In Vivo Imaging System (PerkinElmer, Waltham, MA). Prior to imaging, the instrument was set to collect a minimum of 20,000 photon counts on the auto settings. Imaging parameters were prioritized as exposure time, binning, and ƒ stop. D-luciferin potassium salt (catalogue #MB102, Syd Labs Inc., Natick, MA) was resuspended in phosphate-buffered saline (PBS) to a final concentration of 20 mg/ml. Mice were dosed with 200 μL luciferin substrate intraperitoneally and waited for 10 minutes to allow for distribution of substrate prior to image acquisition. Animals were anaesthetized with 3% isoflurane with a 1 L/min induction chamber flow rate and maintained at 2% isoflurane at a 0.5 L/min imaging chamber flow rate. After the completion of imaging, mice were replaced in the cage in a sternal position and observed for 10-15 minutes until becoming conscious. Endpoint was determined in consulting with a veterinarian, including the following criteria: palpable tumor in the lower abdomen > 1.5 cm in diameter, inability to eat or drink, inability to ambulate (i.e. when animal failed to move when approached), inability to pass urine, or loss of 15% body weight during the course of weekly weighing. If an animal met three out of the five criteria, it would be euthanized for endpoint analysis. Prior to euthanasia, in vivo imaging was performed as described above. Immediately after imaging, the animal was given another dose of D-luciferin and euthanasia was accomplished with CO2 asphyxia and subsequent cervical dislocation. The animal was weighed intact. The entire genitourinary (GU) bloc was dissected out of the carcass, weighed, and imaged in a Petri dish filled with PBS, adjacent to the carcass. The carcass without GU bloc was imaged again from both ventral and dorsal views for additional luciferase signals that were indicative of metastases. Putative metastatic sites were dissected out and imaged to confirm the tissue origin of luciferase signals, in comparison to the imaging of the carcass. The GU bloc and isolated tissues were fixed in 4% paraformaldehyde overnight at 4°C and subsequently placed in 70% ethanol.

Syd Labs公司合成的Syd LabsD(萤）荧光素钾盐 D-luciferin potassium salt MB102产品覆盖各大学科研所和医院里的动物研究以及生物医学研究机构的科学家，已经获得广大科研工作者认可。D-荧光素钾盐（D-luciferin potassium salt）在进口D-荧光素钾盐中价格最低。

武汉多找找科技是Syd Labs中国区合作伙伴，为科研工作者提供优质的产品与服务。Syd Labs  D-luciferin potassium salt MB000102-R70170 or MB102 D-荧光素钾盐价格或其它更多产品参数信息，来自于武汉多找找科技，欢迎大家询单。本产品仅限于科研使用，不得用于人类或动物的诊断或治疗。

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