Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein C-His-Flag Tag | Syd Labs BP003359
(悉得）Syd Labs 货号：BP003359 Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein with the C-His-Flag Tag (食蟹猴 CD3E&CD3D/CD3 ε&CD3 δ 蛋白 C-His-Flag 标签) is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D).
产品参数
| 货号 | BP003359 |
|---|---|
| 产品名称 | Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein C-His-Flag Tag | Syd Labs BP003359 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 物种 | Cynomolgus |
| 来源 | The recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein, (amino acids Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)) fused with a His-Flag tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q95LI5.2(CD3E)&Q95LI8(CD3D) |
| 产品标签 | C-His-Flag |
| 生物素化 | no |
| 确定序列 | Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D) |
| 分子量 | The recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta protein with the His-Flag tag at the C-terminus has a predicted MW of 11.93 kDa (CD3E) and 10.55 kDa (CD3D). Due to glycosylation, the protein migrates to 13-20 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Cynomolgus CD3E&CD3D protein with the C-His-Flag tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CD3 Antibody protein with the C-hFc tag with the EC50 of 3.3 ng/ml determined by ELISA. |
| Form | Liquid |
| 形式 | Supplied as 0.22μm filtered solution in PBS (pH 7.4). |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein performance should be determined experimentally by the investigator. |
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文献
BP003359: Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein with the C-His-Flag Tag (食蟹猴 CD3E&CD3D/CD3 ε&CD3 δ 蛋白 C-His-Flag 标签)
T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
请记住我们的产品信息: 食蟹猴 CD3E&CD3D/CD3 ε&CD3 δ 蛋白 （C-His-Flag 标签）BP003359 Syd Labs（Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein with the C-His-Flag Tag）.