Cynomolgus CD3E/CD3 epsilon Protein C-hFc Tag | Syd Labs BP005557
(悉得）Syd Labs 货号：BP005557 Cynomolgus CD3E/CD3 epsilon Protein with the C-hFc Tag (食蟹猴 CD3E/CD3 ε 蛋白 C-hFc 标签) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asp117.
产品参数
| 货号 | BP005557 |
|---|---|
| 产品名称 | Cynomolgus CD3E/CD3 epsilon Protein C-hFc Tag | Syd Labs BP005557 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
| 物种 | Cynomolgus |
| 来源 | The recombinant Cynomolgus CD3E/CD3 epsilon Protein, (amino acids Gln22-Asp117) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells. |
| Accession | Q95LI5-1 |
| 产品标签 | C-hFc |
| 生物素化 | no |
| 确定序列 | Gln22-Asp117 |
| 分子量 | The recombinant Cynomolgus CD3E/CD3 epsilon protein with the hFc tag at the C-terminus has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Cynomolgus CD3E protein with the C-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Anti-CD3E Antibody protein with the C-hFc tag with the EC50 of 78.8 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Cynomolgus CD3E/CD3 epsilon Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | It is recommended to reconstitute the lyophilized recombinant Cynomolgus CD3E/CD3 epsilon Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Cynomolgus CD3E/CD3 epsilon Protein performance should be determined experimentally by the investigator. |
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文献
BP005557: Cynomolgus CD3E/CD3 epsilon Protein with the C-hFc Tag (食蟹猴 CD3E/CD3 ε 蛋白 C-hFc 标签)
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
请记住我们的产品信息: 食蟹猴 CD3E/CD3 ε 蛋白 （C-hFc 标签）BP005557 Syd Labs（Cynomolgus CD3E/CD3 epsilon Protein with the C-hFc Tag）.
