Cynomolgus B7-H3/CD276 Protein C-His Tag | Syd Labs BP005215
Syd Labs 货号：BP005215 Cynomolgus B7-H3/CD276 Protein with the C-His Tag (食蟹猴 B7-H3/CD276 蛋白 C-His 标签) is expressed from HEK293 with His tag at the C-Terminus.It contains Leu29-Glu465.
产品参数
| 货号 | BP005215 |
|---|---|
| 产品名称 | Cynomolgus B7-H3/CD276 Protein C-His Tag | Syd Labs BP005215 |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276 |
| 物种 | Cynomolgus |
| 来源 | The recombinant Cynomolgus B7-H3/CD276 Protein, (amino acids Leu29-Glu465) fused with a His tag at the C-terminus, was produced in HEK 293 cells. |
| 产品标签 | C-His |
| 生物素化 | no |
| 确定序列 | Leu29-Glu465 |
| 分子量 | The recombinant Cynomolgus B7-H3/CD276 protein with the His tag at the C-terminus has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| 活性 | Immobilized recombinant Cynomolgus B7-H3 protein with the C-His tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-B7-H3 Antibody protein with the C-hFc tag with the EC50 of 11.1 ng/ml determined by ELISA. |
| Form | Lyophilized |
| 形式 | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 制剂 | It is recommended to reconstitute the lyophilized recombinant Cynomolgus B7-H3/CD276 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
| 纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
| 内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
| 运输 | The recombinant Cynomolgus B7-H3/CD276 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.； -20 to -80°C for 3-6 months in unopened state after reconstitution.； 2-8°C for 2-7 days after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| 注意事项 | Conditions of optimal recombinant Cynomolgus B7-H3/CD276 Protein performance should be determined experimentally by the investigator. |
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文献
BP005215: Cynomolgus B7-H3/CD276 Protein with the C-His Tag (食蟹猴 B7-H3/CD276 蛋白 C-His 标签)
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
请记住我们的产品信息: 食蟹猴 B7-H3/CD276 蛋白 （C-His 标签）BP005215 Syd Labs（Cynomolgus B7-H3/CD276 Protein with the C-His Tag）.
