Cell and Tissue Direct PCR Kit
Syd Labs Cell and Tissue Direct PCR Kit MB103-11, Cell and Tissue Direct PCR Kits can be used for both easy and rapid genomic DNA isolation and preparation from animal cells and tissue samples coupled with PCR.
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| 货号 | MB103-11 |
|---|---|
| 产品名称 | Cell and Tissue Direct PCR Kit |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 注意事项 | The cheapest Cell and Tissue Direct PCR Kits and PCR Master Mixes in the market. Free samples of Cell and Tissue Direct PCR Kits and PCR Master Mixes may be available. |
| 线下下单 | 电话: 18162660842微信: duozhaozhao_2017Email: message@sydlabs.comOr 联系我们 |
描述
MB103-11: Cell and Tissue Direct PCR Kit
Background
Cell and Tissue Direct PCR Kit = Rapid Cell and Tissue Genomic DNA Extraction Kit (Catalog No. MB103-10) + 2x PCR PreMix, with dye (Catalog No. MB067-EQ2-100). The kits and reagents are routinely used in mutation detection, SNP genotyping, and target detection in transgenic animals.
Rapid genomic DNA extraction:
Step 1:
A) Mouse tails: Place 2-3 mm mouse tail in a 0.5 ml microcentrifuge tube containing 100 ul solution A.
B) Cultured cells: Add 1×10^4 – 1×10^5 cell suspension in <10 ul to a 0.5 ml microcentrifuge tube containing 100 ul solution A or add 100 ul solution A to a cell pellet that contains 1x10^4 - 1x10^5 cells.
Step 2:
Place the tube in a thermocycler and heat at 95°C for 5 min. Cool the tube to room temperature. Add 10 ul solution B and vortex briefly. Extracted genomic DNA is now ready for use as PCR temperate or for longer term storage at -20°C.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
Please remember our product information: Cell and Tissue Direct PCR Kit: MB103-11 Syd Labs
