Biotinylated Recombinant Human ACE2-His-Avi Protein
Syd Labs Biotinylated Recombinant Human ACE2-His-Avi Protein BP003060, Biotinylated recombinant human ACE2 protein (rhACE2-His and rhACE2-Fc) expressed in HEK 293 cells. Immobilized recombinant SARS-CoV-2 Spike S RBD. Dose response curve for the biotinylated recombinant human ACE2 protein with the EC50 of 0.1 ug/ml determined by ELISA.
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| 货号 | BP003060 |
|---|---|
| 产品名称 | Biotinylated Recombinant Human ACE2-His-Avi Protein |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | Angiotensin I Converting Enzyme 2, hACE2, rhACE2, ACE-2, hACE-2, rhACE-2 |
| 来源 | Biotinylated recombinant human ACE2 protein (Gln18-Ser740) was expressed in mammalian cells with a His tag and Avi at the C-terminus. |
| 形式 | The biotinylated recombinant human ACE2-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| 纯度 | > 95% by PAGE under reduced condition, and SEC-HPLC. |
| 内毒素 | Less than 1 EU per ug by the LAL method. |
| 运输 | The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below. |
| 注意事项 | Both His-tagged and Fc-fused recombinant human and cyno ACE2 protein (rhACE2-His, rhACE2-Fc, and rcACE2-His) are available with an Avi site, which is for biotinylation. Please ask for a quote if you need protein mutants or a protein with different modification or components. |
| 线下下单 | 电话: 18162660842微信: duozhaozhao_2017Email: message@sydlabs.comOr 联系我们 |
描述
BP003060: Biotinylated Recombinant Human ACE2-His-Avi Protein
Background
Source: Biotinylated recombinant human ACE2 protein (Gln18-Ser740) was expressed in mammalian cells with a His tag and Avi at the C-terminus.
Accession: Q9BYF1
Predicted molecular mass: 86.5 kDa. Due to glycosylation, the biotinylated recombinant human ACE2 protein migrates to 95-110 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Activity: Immobilized recombinant SARS-CoV-2 (2019-nCoV) Spike S RBD at 1 ug/ml (100 ul/well). Dose response curve for the biotinylated recombinant human ACE2 protein with the EC50 of 0.1 ug/ml determined by ELISA. Conditions of optimal biotinylated recombinant Human ACE2 protein (rhACE2-His, rhACE2-Fc, and rcACE2-His) performance should be determined experimentally by the investigator.
Formulation: The biotinylated recombinant human ACE2-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.
Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension. The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidylcarboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services
Please remember our product information: Biotinylated Recombinant Human ACE2-His-Avi Protein: BP003060 Syd Labs