Biotinylated Mouse GFRAL/GFR alpha-like Protein N-His-Avi Tag | Syd Labs BP004085

Biotinylated Mouse GFRAL/GFR alpha-like Protein BP004085 Syd Labs

Biotinylated Mouse GFRAL/GFR alpha-like Protein N-His-Avi Tag | Syd Labs BP004085

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(悉得)Syd Labs 货号:BP004085 Biotinylated Mouse GFRAL/GFR alpha-like Protein with the N-His-Avi Tag (生物素化小鼠 GFRAL/GFR α 样蛋白 N-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu350.

SKU: BP004085 分类: ,

产品参数

货号 BP004085
产品名称Biotinylated Mouse GFRAL/GFR alpha-like Protein N-His-Avi Tag | Syd Labs BP004085
供货商名称 Syd Labs, Inc.
品牌名 Syd Labs
物种 Mouse
来源 The recombinant Biotinylated Mouse GFRAL/GFR alpha-like Protein, (amino acids Gln20-Glu350) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Accession Q6SJE0-1
产品标签 N-His-Avi
生物素化 yes
确定序列 Gln20-Glu350
分子量 The recombinant Biotinylated Mouse GFRAL/GFR alpha-like protein with the His-Avi tag at the N-terminus has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Biotinylated Mouse GFRAL protein with the N-His-Avi tag at 0.5 ug/ml (100 ul/Well) on streptavidin (5 ug/ml) precoated plate. Dose response curve for the recombinant Mouse GDF15 protein with the N-hFc tag with the EC50 of 2.4 ng/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Biotinylated Mouse GFRAL/GFR alpha-like Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Biotinylated Mouse GFRAL/GFR alpha-like Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
注意事项 Conditions of optimal recombinant Biotinylated Mouse GFRAL/GFR alpha-like Protein performance should be determined experimentally by the investigator.
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文献

BP004085: Biotinylated Mouse GFRAL/GFR alpha-like Protein with the N-His-Avi Tag (生物素化小鼠 GFRAL/GFR α 样蛋白 N-His-Avi 标签)

 

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

请记住我们的产品信息: 生物素化小鼠 GFRAL/GFR α 样蛋白 (N-His-Avi 标签)BP004085 Syd Labs(Biotinylated Mouse GFRAL/GFR alpha-like Protein with the N-His-Avi Tag).